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Tag Archives: NGS
Signal Boosting a Comprehensive Review of eDNA and Metabarcoding Studies
Everything is meta these days – metabarcoding, metagenomics, and now meta blog posts that are reviews of reviews. Much like every ecologist at least dabbles in the molecular world, so most of those predisposed to molecular ecology and population genetics … Continue reading →
Posted in bioinformatics, community ecology, DNA barcoding, metagenomics, methods, microbiology, next generation sequencing, population genetics
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Tagged barcode tags, barcoding, bioinformatics, eukaryotes, Illumina, metagenomics, methods, microbial communities, microbial community analysis, microbiome, NGS
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Major new microbial groups expand diversity and alter our understanding of the tree of life
I still believe in revolutions. And sometimes they just happen, almost unnoticed. One such revolution happened on a boring 11th of April 2016 when Laura Hug et al. published their new tree of life in the journal of Nature Microbiology. … Continue reading →
Posted in bioinformatics, community ecology, evolution, genomics, metagenomics, microbiology, next generation sequencing, phylogenetics
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Tagged assembly, bioinformatics, Evolution, genome, genomics, metagenomics, NGS, phylogenetics
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We have the technology. Is sequencing getting better, smaller, faster?
Okay, I know some version of the phrase “recent developments in rapid and affordable sequencing have made blah blah blah possible…” is something you’ve probably read 10,000 times. However, third-generation sequencing platforms have turned out to be pretty darn astounding. … Continue reading →
Posted in bioinformatics, evolution, fieldwork, genomics, methods, next generation sequencing
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Tagged genomics, MinION, NGS, third-generation sequencing
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1 Comment
Right reads, wrong index? Concerns with data from Illumina's HiSeq 4000
Commanding around a 70% share of a 1.3 billion USD market, Illumina is the major player in next-generation sequencing (NGS) technology. More likely than not, if you’re a molecular ecologist working with NGS data, you’ve run your samples on a … Continue reading →
Posted in genomics, next generation sequencing, RNAseq, technical, transcriptomics
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Tagged high throughput sequencing, HiSeq 4000, Illumina, next gen sequencing, NGS
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5 Comments
The Great Migration and African-American Genomic History
Over 45 million African-Americans share a recent common history largely shaped by “The Great Migration” (1910-1970) from out of the Southern United States. And yet, the admixture history of the African-American community, and its consequences for public health are little … Continue reading →
Posted in genomics, population genetics, United States
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Tagged Evolution, gene flow, Illumina, NGS, population genetics, population structure
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Results of the Molecular Ecologist's Survey on High-Throughput Sequencing
Some days ago, we asked our readers to fill in a survey (now closed) on your use of high-throughput sequencing techniques. We got a lot of responses, a total of 260 people filled in the form. Thank you! Here are the results of your answers. The … Continue reading →
Posted in bioinformatics, genomics, next generation sequencing
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Tagged molecular ecologist, NGS, NGS Field Guide, Sequencing, survey
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1 Comment
The Molecular Ecologist's Survey on High-Throughput Sequencing
Last week we updated the Field Guide to Next Generation DNA Sequencers to better reflect the sequencing techniques of 2016. The NGS Field Guide is one of the most popular resources on the Molecular Ecologist web site, but we don’t know much about how our readers use … Continue reading →
Posted in bioinformatics, genomics, methods, next generation sequencing
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Tagged molecular ecologist, NGS, NGS Field Guide, Sequencing, survey
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2 Comments
The Truth
Spoiler Alert: I’ve taken plenty of care to try and not spill the beans on any plotlines, but you have been forewarned that there may be some aspects of the science that’s discussed on the show that I attempt to … Continue reading →
Posted in genomics, next generation sequencing, population genetics, TV shows
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Tagged data visualization, gene flow, genomics, NGS, opinion, population genetics
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The 2016 Workshop on Genomics summary
I recently had the pleasure to spend two and a half weeks in the beautiful medieval town of Český Krumlov, Czech Republic. The reason was the popular Workshop on Genomics that was running and I was one of the TAs … Continue reading →
Posted in bioinformatics, genomics, next generation sequencing
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Tagged bioinformatics, genomics, NGS, RADseq, RNAseq, unix, workshop
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Bees brought to their knees
As regular readers of TME will have read, this past summer was a whirlwind of sampling in which I took the briefest of holidays in the Southwest of England before attending the European Phycological Congress (read about the congress here and here). … Continue reading →
Posted in bioinformatics, Coevolution, evolution, genomics, next generation sequencing
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Tagged bees, Marine Biological Association, NGS, pipelines, RNA, viruses
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