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Tag Archives: methods
Metabarcoding for every body, every habitat, every time
The immediate reason why I wanted to write about Boosting DNA metabarcoding for biomonitoring with phylogenetic estimation of operational taxonomic units’ ecological profiles is its usefulness for the scientific community and the effort of the authors to make their study reproducible. … Continue reading
Signal Boosting a Comprehensive Review of eDNA and Metabarcoding Studies
Everything is meta these days – metabarcoding, metagenomics, and now meta blog posts that are reviews of reviews. Much like every ecologist at least dabbles in the molecular world, so most of those predisposed to molecular ecology and population genetics … Continue reading
Posted in bioinformatics, community ecology, DNA barcoding, metagenomics, methods, microbiology, next generation sequencing, population genetics
Tagged barcode tags, barcoding, bioinformatics, eukaryotes, Illumina, metagenomics, methods, microbial communities, microbial community analysis, microbiome, NGS
1 Comment
Unbalanced population sampling and STRUCTURE
The utility and intuition offered by the program STRUCTURE, and more generally, the ‘admixture’ model of Pritchard et al. (2000) are unquestioned – with tens of thousands of citations, it retains its lead among the most popular population genetics software. … Continue reading
Posted in bioinformatics, genomics, howto, methods, population genetics, software, STRUCTURE
Tagged gene flow, methods, population structure
1 Comment
Making ecology “count”: a review of the why and how of molecular ecology
It’s likely that everyone has been asked by either a friend or family member “What do you do?” Which, depending on what level of detail you shoot for, might be relatively straight forward. The follow-up question, however, can be a … Continue reading
Posted in bioinformatics, community, community ecology, metagenomics
Tagged genomics, methods, omics, review
1 Comment
When your programming may be inadequate to the task: new options for metagenome analysis
There’s a lot of data in the form of metagenomes out there, and picking apart those mountains of data to uncover meaningful results is difficult. Recently, we received a suggestion from a reader to discuss a recent program (CLARK-S) developed … Continue reading
Posted in bioinformatics, community ecology, metagenomics, methods
Tagged CLARK-S, DESMAN, fine-scale variation, metagenomics, methods, OMICtools
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The trouble with PCR duplicates
The sequencing center just sent your lane of Illumina data. You’re excited. Life is great. You begin to process the data. You align the data. You check for PCR duplicates. 50 percent. Half of your data is garbage. Everything is … Continue reading
Posted in bioinformatics, genomics, methods, next generation sequencing
Tagged genomics, methods, PCR duplicates, RADseq
6 Comments
Catching evolution in the act with the Singleton Density Score
A recent study led by Jonathan K. Pritchard at Stanford University brought a media storm with catchy headlines in both of the flagship scientific outlets Nature and Science News. Aside from highlighting the question of preprints without peer review being … Continue reading
Posted in methods, mutation, population genetics, selection
Tagged human evolution, methods, SDS, selection
1 Comment
Geographical Heat Maps in R
I go crazy for fancy data visualizations in R, and a figure in a recent publication has had me wondering if there is an easy way to incorporate density distributions (or as in their case, a distribution of f4 statistics, … Continue reading
Genomics of Hybridization – Part II, Top three of 2015
Death Valley pupfishes (Cyprinodon) are among the most endangered vertebrates on earth, with small inbred populations, with heavy risks of extinction in extreme environments. Martin et al. (2016) in a recent publication quantify diversity and adaptability in a very small population … Continue reading
On false positives in Isolation with Migration analyses
The IM suite of tools (IM, IMa, IMa2, IMa2p, etc.) are used widely by molecular ecologists at large for the analyses and estimation of ancestral demography under an Isolation with Migration (IM) model. However, these tools come with fundamental assumptions … Continue reading
Posted in evolution, genomics, howto, IMa2, methods, Molecular Ecology views, natural history, population genetics, software, theory
Tagged Evolution, gene flow, genomics, IM, isolation with migration, methods, migration, population genetics
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