Category Archives: methods

Snapshots of Biodiversity: eDNA as a methodology for species detection

Nicole Conner wrote this post as a project for Stacy Krueger-Hadfield’s Conservation Genetics course at the University of Alabama at Birmingham.  She is a Master’s student in Dr. Thane Wibbels’ lab where she is developing new protocol to detect diamondback terrapins off … Continue reading

Posted in bioinformatics, blogging, citizen science, community, conservation, DNA barcoding, ecology, evolution, fieldwork, metagenomics, natural history | Tagged , , , , | Leave a comment

Genetics of Returning Turtles

Amy Bonka wrote this post as a project for Stacy Krueger-Hadfield’s Conservation Genetics course at the University of Alabama at Birmingham. Amy grew up in Florida, completed a BS in Biology with a concentration in Marine Science and Chemistry as well … Continue reading

Posted in blogging, citizen science, conservation, DNA barcoding, ecology, evolution, fieldwork, methods, natural history, Science Communication | Tagged , , , , | Leave a comment

Conference catch-up: The many colors of snow

Red snow … watermelon snow … green snow … did you know that snow came in so many different colors? I had never heard of watermelon ice (#🍉❄) until a talk given by Robin Kodner from Western Washington University at … Continue reading

Posted in adaptation, bioinformatics, citizen science, community ecology, evolution, fieldwork, mating system, microbiology, natural history, phylogenetics, phylogeography, population genetics, selection, speciation, transcriptomics | Tagged , , , , , , , , , , , , | Leave a comment

For flexible eDNA analysis, just capture whatever you want

Environmental DNA sampling for multi-taxa species detection (i.e., the inference of species presence from genetic material in the environment) has been a hot topic lately. Some of the most exciting recent work has used high-throughput sequence (HTS) to simultaneously screen … Continue reading

Posted in community ecology, DNA barcoding, metagenomics, methods | Tagged , | 1 Comment

What is DAS? A new tool to recover genomes from metagenomes

There are a lot of data out there, and if you haven’t already noticed the ‘omics train has steadily stayed its path through the fruitful (but challenging) world of metagenomics. Metagenomics offers the chance to unravel complex microbial communities without … Continue reading

Posted in bioinformatics, community ecology, genomics, metagenomics, methods, microbiology, next generation sequencing | Tagged , , , | Leave a comment

Best practices in sample naming

Wherein I try to save me from myself Let’s imagine a young scientist, bursting to the seems with enthusiasm and schemes to uncover the secrets of the biological world. Everything is new and she learns as she goes! Let’s call … Continue reading

Posted in data archiving, fieldwork, howto, methods | Tagged , | 2 Comments

Signal Boosting a Comprehensive Review of eDNA and Metabarcoding Studies

Everything is meta these days – metabarcoding, metagenomics, and now meta blog posts that are reviews of reviews. Much like every ecologist at least dabbles in the molecular world, so most of those predisposed to molecular ecology and population genetics … Continue reading

Posted in bioinformatics, community ecology, DNA barcoding, metagenomics, methods, microbiology, next generation sequencing, population genetics | Tagged , , , , , , , , , , | 1 Comment

Retrieving a million sequences and avoiding primer bias, a new method that might have it all

We have come a long way since the early days when sequencing was a breakthrough method initially used to identify uncultured microbes from the environment. It is now been almost three decades, in fact, since the first microbial 16S rRNA … Continue reading

Posted in bioinformatics, evolution, metagenomics, methods, microbiology, next generation sequencing | Tagged , , | Leave a comment

We have the technology. Is sequencing getting better, smaller, faster?

Okay, I know some version of the phrase “recent developments in rapid and affordable sequencing have made blah blah blah possible…” is something you’ve probably read 10,000 times. However, third-generation sequencing platforms have turned out to be pretty darn astounding. … Continue reading

Posted in bioinformatics, evolution, fieldwork, genomics, methods, next generation sequencing | Tagged , , , | 1 Comment

Of Of Mice and Men: High school English class lives on in scientific paper titles

Writing titles for scientific papers is hard. The title is the one element of the paper everyone reads if they so much as skim a journal’s table of contents e-mail. These days, you also want something that’ll fit in a … Continue reading

Posted in just for fun, methods, science publishing | Tagged , , , , , , , | 3 Comments