Tag Archives: NGS

And who made your beer?

In the spirit of it being almost Friday, and while we’re on the topic of your favorite beverages – perhaps wine puts you to sleep, couldn’t care less where it came from, but prefer the bitterness of lager beers at your … Continue reading

Posted in adaptation, evolution, genomics, natural history, next generation sequencing, phylogenetics, population genetics, selection, speciation, yeast | Tagged , , , , | 2 Comments

Who came first – the Paleo- or Native American?

In yet another infamous Science vs Nature race, two studies published this Tuesday toss more cans of worms at the ongoing debate about the founding of the Americas – with disparate findings. Uh oh. Skoglund et al. Nature (2015) Genetic … Continue reading

Posted in genomics, next generation sequencing, Paleogenomics, population genetics | Tagged , , , , | 2 Comments

The brave new world of environmental genomics

A new special issue of Heredity reflects on the recent advances in environmental genomics (see other posts about eDNA here and here) and highlights the ways NGS can aid in characterizing complex biological systems. The cryptic, as well as the rare but active … Continue reading

Posted in bioinformatics, Coevolution, DNA barcoding, evolution, genomics | Tagged , , , | 1 Comment

The Ust'-Ishim Genome

This year has been monumental in pulling together several interesting pieces in the human evolution out of Africa puzzle (Lazaridis et al., Ruiz-Linares et al., Skoglund et al., Huerta-Sanchez et al., Jeong et al., Pickrell et al., Raghavan et al., … Continue reading

Posted in genomics, mutation, Paleogenomics, population genetics | Tagged , , | 4 Comments

Dramatically reducing sequencing error via Duplex Tag sequencing

An exciting new study was published in PNAS last month, an open access paper entitled “Detection of ultra-rare mutations by next-generation sequencing”. This new method has the potential to open up a new frontier in Next-gen sequencing bioinformatics, since it allows … Continue reading

Posted in methods, next generation sequencing | Tagged , , , , | 5 Comments

Riding the Elephant

I recently received my first batch of reads from a single paired-end lane run on an [Illumina Hi-Seq](http://www.illumina.com/systems/hiseq_2000.ilmn) instrument. This batch totaled about 20 billion basepairs of DNA sequence, and the associated data files a combined 55.4 gigs of text. … Continue reading

Posted in bioinformatics, next generation sequencing, software | Tagged , , , , | 9 Comments