Category Archives: technical

Right reads, wrong index? Concerns with data from Illumina's HiSeq 4000

Posted on 11 Apr, 2017 by Ethan Linck

Commanding around a 70% share of a 1.3 billion USD market, Illumina is the major player in next-generation sequencing (NGS) technology. More likely than not, if you’re a molecular ecologist working with NGS data, you’ve run your samples on a … Continue reading →

Posted in genomics, next generation sequencing, RNAseq, technical, transcriptomics | Tagged high throughput sequencing, HiSeq 4000, Illumina, next gen sequencing, NGS | 5 Comments

Handling microbial contamination in NGS data

Posted on 6 Jan, 2017 by Ethan Linck

Until recently, I had given little thought to the potential for unwanted microbial contamination in high throughput sequence data. I suspect that if you’re a molecular ecologist who doesn’t primarily study microbes or work with ancient DNA, you’re in a … Continue reading →

Posted in bioinformatics, genomics, howto, next generation sequencing, technical | Tagged bioinformatics, contamination, microbes, microbial contamination | Leave a comment

A note about e-mail subscriptions

Posted on 4 Apr, 2016 by Jeremy Yoder

If you recieve The Molecular Ecologist by e-mail, you’re far from alone — we have almost 650 subscribers recieving e-mailed posts via the Feedburner service. (How do you know if you’re one of them? You are if you’re reading this … Continue reading →

Posted in housekeeping, technical | Tagged e-mail subscriptions | Leave a comment

Want to see us in your Facebook News Feed? You should probably do this one weird thing.

Posted on 24 Mar, 2014 by Jeremy Yoder

A whole lot of folks—875!—have “liked” the Molecular Ecologist page on Facebook, which ought to mean that all those people see new posts from the site right in their Facebook News Feed. But we’ve found that our Facebook posts are … Continue reading →

Posted in housekeeping, technical | Leave a comment

Discussion, now with Disqus

Posted on 15 Mar, 2013 by Jeremy Yoder

Readers may have noticed that our comments forms have changed—because we’ve installed the Disqus commenting platform for this site. (Old comments are currently being imported into the new system, which should be done within 24 hours.) Disqus offers some nice … Continue reading →

Posted in housekeeping, technical | Leave a comment

Syndication, really simple (and, how will you subscribe?)

Posted on 14 Mar, 2013 by Jeremy Yoder

With the recent announcement that Google Reader’s days are numbered, it’s occurred to me to clean up the options for RSS (really simple syndication) subscriptions here at The Molecular Ecologist. To that end, I’ve installed a shiny new Feedburner RSS … Continue reading →

Posted in housekeeping, technical | 5 Comments