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Tag Archives: museum genomics
Move or adapt to changing climate? These chipmunks have had to do both
Climate change threatens to land many, many species in conditions for which they’re not adapted — too warm, too dry, too stormy, too flood-prone — and traditionally the ways that living things might respond to this are framed as a … Continue reading →
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DNA extraction for PacBio sequencing
PacBio is emerging as the favoured sequencing approach for assembling high-quality reference genomes. But the big issue with PacBio sequencing is that to get long sequence reads you need to start with high molecular weight DNA. For my first foray … Continue reading →
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Posted in genomics, next generation sequencing, Uncategorized
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Tagged DNA extraction, museum genomics, PacBio, quality control
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On hyRAD-X, another option for museum genomics
Last year, I profiled Suchan et al.’s “hyRAD” method for reduced-representation genome sequencing of degraded sources of DNA using RAD probes. While it’s too early to say whether hyRAD will be widely used by molecular ecologists looking to integrate historic … Continue reading →
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Posted in genomics, methods, natural history, next generation sequencing, phylogenetics, phylogeography, population genetics, RNAseq, selection, transcriptomics
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Tagged hyRAD, hyRAD-X, museum genomics, RNAseq
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hyRAD and museum genomics
While the RAPTURE may have arrived, the development of novel restriction digest-based library prepartation techniques — and portmanteaus — continues unabated. In a paper published in PLoS ONE last month (and previously available as a preprint on bioRxiv), Tomasz Suchan … Continue reading →
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Posted in genomics, methods, natural history, next generation sequencing, phylogenetics, phylogeography, population genetics
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Tagged ddRADseq, hybridization RAD, hyRAD, museum genomics, RADseq, sequence capture
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