2014 NGS Field Guide – Table 3c – Error rates

Table 3c. Error rates for announced & commercially available DNA sequencing platforms. All Error rates are in percentage. Percentage of errors per base within single reads of the maximum length. As explained in Glenn (2011), error rates among platforms are not exactly comparable. The reported Ion Torrent rates range from 0.46% to 2.4%. Final Error rates for SOLiD are from reads with bases consistent on double or triple sequencing only. Final Error rate for PacBio applies only to consensus sequencing for three independent reads of the same template. For Illumina, errors at ≤0.1% is achieved for ≥ 75-85% of bases. In general, Illumina keeps similar error rate criteria, but extends the maximum read length with new versions of their chemistry. See Glenn (2011) for additional details. See Ross et al. (2013, Genome Biology 14:R51) for additional current information on bias and error rates.

Instrument

Primary Errors

Single-pass Error Rate (%)

Final Error Rate (%)

3730xl (capillary)

substitution

0.1-1

0.1-1

454 All models

indel

1

1

Illumina All Models

substitution

~0.1

~0.1

Ion Torrent – all chips

Indel

~1

~1

SOLiD – 5500xl

A-T bias

~5

≤0.1

Oxford Nanopore

deletions

≥4*

4*

PacBio RS

Indel

~13

≤1

* Information based on company sources alone from early 2012 (independent data not yet available as of Feb 2014); it is not clear if the 4% error rate reported by Oxford Nanopore refers to a single-pass rate or is what is achieved after reading both strands & producing a consensus sequence; nor is it clear what the error rate will be for instruments when they are released.