Table 3a. Instrument purchase cost, additional instrument costs, and service agreement costs for common commercially next generation DNA sequencing platforms available in 2016. All costs are list price in thousands of US dollars.
|Instrument||Purchase Cost||Additional Instruments a||Service Contract b|
|Illumina NextSeq 500
|Illumina HiSeq 2500||$690||$55c||$74.5 + $6|
|Illumina HiSeq 4000||$900||$55c||$81 + $6|
|Illumina HiSeq X *||$1,200||$55c||$92.7 + $6|
|Ion Torrent – PGM (314/316/318 chips)||$49||$18/32 d||$4.3-8.5/9.9 e|
|Ion Torrent – Proton||$224||$19 f||$19.9/32.8 g|
|Ion Torrent – S5||$65||$19
|Ion Torrent – S5xl||$150||$19
|Oxford Nanopore MinION||$1 h||$0||$0|
|Oxford Nanopore PromethION||$75 i||$0||$0|
|ThermoFisher SOLiD – 5500xl||$251||$54 j||$44.4 k
a Costs include specific instruments when required by the instrument manufacturer (e.g., for emPCR), but do not include general purpose and library preparation equipment for construction of libraries. Many labs will need additional general purpose instruments.
b Annual maintenance agreements for “basic” on-site service of sequencer + any required additional instruments.
c Cost of cBot (required for high output flow cells and loading separate libraries onto each lane of rapid run flow cells; not needed to load a single pool onto both lanes of rapid run flow cells); $6k service contract added to all HiSeqs.
d Required $16.5k Ion Torrent server for conversion of raw signals to base calling; $1k ULTRA-TURRAX® Tube Drive; Argon gas tank (<$0.5k); / cost with $14.5k OneTouch
e Maintenance contract for sequencer alone / cost including Server and OneTouch
f Ion OneTouch 2.0 ($19k); Argon gas tank with valves ($0.5k); server now bundled with instrument / training is optional ($3.3k at Life Tech campus; $8.5k at customer site)
g Proton alone / Proton + Server + OneTouch.
h Instrument access fee (deposit) – MinION includes reagents for 2 runs.
i Instrument access fee (deposit) – eliminated with consumable purchases
j Includes EZ Bead ePCR automation and required UPS; a Covaris ($45K) is also required but is not included to facilitate comparisons (usually part of general purpose library preparation equipment).
k Includes EZ Bead, Covaris, and one preventative maintenance.
*Minimum of 5 instruments must be purchased; price drops to $1M each when purchasing ≥10.