Table 3a. Instrument purchase cost, additional instrument costs, and service agreement costs for commercially available DNA sequencing platforms in 2012. All costs are list price in thousands of US dollars.
|Instrument||Purchase Cost||Additional Instrumentsa||Service Contractb|
|454 GS Jr. Titanium||$108||$16||$12.6|
|454 FLX to FLX+ upgrade||$29.5||–||–|
|Ion Torrent – PGM (314/316/318 chips)||$49||$18/32c||$7.5/9.9d|
|Ion Torrent – Proton (chips I, II, III)||$224||$19.5/3.3-8.5e||$22.4f|
|SOLiD – 5500xl||$251||$54g||$44.4h|
|Illumina MiSeq upgrade||$0||–||–|
|Illumina HiSeq 1000||$560||$55i||$62|
|Illumina HiSeq 1000 to 2000 upgrade||$175||–||–|
|Illumina HiSeq 2000||$690||$55i||$75.9|
|Illumina HiSeq 2000 to 2500 upgrade||$50||–||–|
|Illumina HiSeq 2500||$690||$55h||$75.9|
|Oxford Nanopore minION||$0k||$0||$0|
|Oxford Nanopore GridION 2000||?$30?l||?||?|
|Oxford Nanopore GridION 8000||?$30?l||?||?|
a Does not include general purpose and library preparation equipment (e.g., Covaris [$45k], Agilent bioanalyzer [$18k], thermal cyclers, general purpose centrifuges, MilliQ water, etc.), but includes bead counters for emPCR (up to $20k), TissueLysers or similar for emPCR (up to $10k), specialty centrifuges, etc. when required by the instrument manufacturer. Many labs will need additional general purpose instruments.
b Annual maintenance agreements include on-site service, but do not include extra premiums for the fastest available service
c Required $16.5k Ion Torrent server for conversion of raw signals to base calling; $1k ULTRA-TURRAX®Tube Drive; Argon gas tank (<$0.5k); / cost with $14.5k OneTouch
d Maintenance contract for PGM alone / cost including Server and OneTouch
e Required $75k Ion Torrent server for conversion of raw signals to base calling; Argon gas tank with valves ($0.5k); $14.5k OneTouch
f Maintenance contract for Proton alone / cost including Server and OneTouch
g Includes EZ Bead ePCR automation and required UPS; a Covaris ($45K) is also required but is not included to facilitate comparisons (because one is usually bought with any of the other sequencing systems)
h Includes EZ Bead, Covaris, and one preventative maintenance.
i Cost of cBot (required). Additional instruments for library prep needed (Covaris, etc., similar to 454 FLX)
j Cost of cBot and Paired end module (required for GAIIx)
k minION does not require a separate instrument to operate, it simply plugs into a computer’s USB port & feeds data into the computer.
l Oxford Nanopore has not released a target price for the GridION systems, but this number has been mentioned by multiple sources.