2011 NGS Field Guide – Table 3a – Instrument costs

Table 3a. Instrument purchase cost, additional instrument costs, and service agreement costs for commercially available DNA sequencing platforms in 2011.  All costs are list price in thousands of US dollars.

N.B. If you think that these data are inaccurate or out of date, please post a comment below and we’ll attempt to include it in our next update (currently scheduled for May 2012).

Instrument Purchase Cost Additional Instruments a Service Contract b
3730xl (capillary) $376 $19.8
454 GS Jr. Titanium $108 $16 $12.6
454 FLX Titanium $500 $30 $50.0
454 FLX+c $29.5 $30 $50.0
PacBio RS $695 $85.0
Ion Torrent – 314 chip $ 49.5 $18d $7.5
Ion Torrent – 316 chip $49.5 $18d $7.5
Ion Torrent – 318 chip $49.5 $18d $7.5
SOLiD – 4 $475 $54e $38.4
SOLiD – 5500 $349 $54e $29.0
SOLiD – 5500xl $595 $54e $38.4
Illumina MiSeq $125 $12.5
Illumina HiScanSQ $405 $55f $41.5
Illumina GAIIx $250 $100g $44.5
Illumina HiSeq $560h $55f $62.0
Illumina HiSeq 2000 $690 $55f $75.9

a Does not include general purpose and library preparation equipment (e.g., Covaris [$45k], Agilent bioanalyzer [$18k], thermal cyclers, general purpose centrifuges, MilliQ water, etc.), but includes bead counters for emPCR (up to $20k), TissueLysers or similar for emPCR (up to $10k), specialty centrifuges, etc. when required by the instrument manufacturer. Many labs will need additional general purpose instruments.

b Annual maintenance agreements include on-site service, but do not include extra premiums for the fastest available service.

c Upgrade to the 454 FLX instrument

d Required $16.5k Ion Torrent server for conversion of raw signals to base calling; $1k ULTRA-TURRAX® Tube Drive; Argon gas tank (<$0.5k)

e Includes EZ Bead ePCR automation and required UPS; a Covaris ($45K) is also required but is not included to facilitate comparisons (because one is usually bought with any of the other sequencing systems)

f Cost of cBot (required). Additional instruments for library prep needed (Covaris, etc., similar to 454 FLX)

g Cost of cBot and Paired end module (required for GAIIx)

h HiSeq 1000 is upgradable to HiSeq 2000 for $175k

~ = Indicates a likely value based on unpublished information available in February 2011 (i.e., author speculation)

* Information based on company sources alone (independent data not yet available); also applies to Illumina TruSeq v3 chemistry