2011 NGS Field Guide – Table 2b – Costs/run, cost/MB, minimum costs

Table 2b. Comparison of costs for all commercially available 2nd and 3rd generation sequencing platforms, sorted by cost/MB.

N.B. If you think that these data are inaccurate or out of date, please post a comment below and we’ll attempt to include it in our next update (currently scheduled for May 2012).

Instrument Reagent Cost/runa Reagent Cost/MB Minimum Unit Cost (% run)b
3730xl (capillary) $96 $1500 $6 (1%)
Ion Torrent – ‘314’ chip $500 <$50 ~$750 (100%)
454 GS Jr. Titanium $1100 $22 

 

$1500 (100%)
Starlight * ? ? ?
PacBio RS $110-1700c $11-340 ?
454 FLX Titanium $6,200 $12.4 $2000 (10%)
454 FLX+d $6,200 $7 $2000 (10%)
Ion Torrent – ‘316’ chip * 

 

$750 <$7.5 ~$1000 (100%)
Helicose N/A NA $1100 (2%)
Ion Torrent – ‘318’ chip * 

 

~$925 ~$0.93 

 

~$1200 (100%)
Illumina MiSeq * $750 $0.74 ~$1000 (100%)
Illumina iScanSQ $10,220 $0.20 $3000 (14%)
Illumina GAIIx $11,524 $0.12 $3200 (14%)
SOLiD – 4 $8,128 <$0.11 $2500 (12%)
Illumina HiSeq 1000 $10,220 $0.10 $3000 (12%)
Illumina HiSeq 2000 $20,120f $0.10 $3000 (6%)
SOLiD – 5500 (PI) * $6,101 <$0.08 $2000 (12%)
SOLiD – 5500xl (4hq) * $10,503f <$0.07 $2000 (12%)
Illumina HiSeq 2000 – v3g* $23,470 f ≥$0.04 ~$3500 (6%)

aIncludes all stages of sample prep. for a single sample (i.e., library prep through sequencing; capillary = sequencing only)

bTypical full cost (i.e., including labor, service contract, etc.) of the smallest generally available unit of purchase at an academic core lab provider for the longest available read (and percentage of reads relative to a full run, rounded to the nearest whole percentage)

cLibrary preps cost from $200-$1600 per sample, which can be used for 20 to 2 runs (circular consensus to strobed reads, respectively).

dUpgrade of the FLX instrument, due out summer 2011

eInstruments and reagents are no longer sold; services are available for any organism.

fMore reads are obtained than is needed from any single sample within most experiments, but the value illustrates the costs.

gTruSeq v3 reagents & software*, reads and yield are half for HiSeq1000.

? = Detail not yet available

  • Information based on company sources alone (independent data not yet available)

~ = Indicates a likely value based on unpublished information available in May 2011 (i.e., author speculation)