Tag Archives: 16S rRNA

Retrieving a million sequences and avoiding primer bias, a new method that might have it all

Posted on 19 Jan, 2018 by Kelle Freel

We have come a long way since the early days when sequencing was a breakthrough method initially used to identify uncultured microbes from the environment. It is now been almost three decades, in fact, since the first microbial 16S rRNA … Continue reading →

Posted in bioinformatics, evolution, metagenomics, methods, microbiology, next generation sequencing | Tagged , , | Leave a comment

When microbes can move mountains, studying microbial communities on glaciers

Posted on 18 Jan, 2017 by Kelle Freel

Bacteria are amazing, and as a recent article by Ambrosini and colleagues reminds us, they are quite literally, just about everywhere. Before reading this article, I have to admit, I was a little rusty on my definition of cryoconite holes, … Continue reading →

Posted in community ecology, microbiology, Molecular Ecology, the journal | Tagged , , | Leave a comment

Respect the old but seek out the new: Direct 16S rRNA-seq from bacterial communities

Posted on 2 Sep, 2016 by Kelle Freel

I think it’s fair to say that it’s an ongoing struggle to figure out what the heck microbes are doing in their natural environments, and who those microbes are. Clearly, there is no silver bullet that gives us all the … Continue reading →

Posted in community ecology, microbiology, RNAseq | Tagged , , , | 1 Comment