Category Archives: next generation sequencing

The not so singular process of hybridization

What, if anything, are hybrids? Zach Gompert and Alex Buerkle ask this question in a special issue in Evolutionary Applications. Hybrids occur when unrelated individuals mate, but how distant do the taxa need to be to constitute a cross? The varied … Continue reading

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Posted in bioinformatics, conservation, domestication, evolution, genomics, natural history, next generation sequencing, plants | Tagged , , , | Leave a comment

Steelhead in a random forest: identifying the genetic basis of migration

Genome-wide association studies (GWAS) have been quite successful in identifying variants associated with various phenotypes (I suppose there is some debate surrounding this statement. For an interesting, if dated, discussion look here). While most of this work was originally conducted … Continue reading

Posted in association genetics, bioinformatics, conservation, genomics, next generation sequencing | Leave a comment

Poorly updated databases will affect your results

If you’re anything like me, your research is heavily dependent on the many wonderful database resources available online. NCBI, UniProtKB, Ensembl, Swiss-Prot, EMBL-EBI, and many other sites and organizations offer highly useful (and often curated) molecular information. Can you imagine … Continue reading

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RADseq and missing data: some considerations

Unlike Sanger sequencing, where loci are directly targeted for each individual and sequencing errors are relatively rare, massively multilocus datasets from next generation sequencing platforms are characterized by large amounts of missing data. This is particularly true for restriction digest … Continue reading

Posted in bioinformatics, genomics, methods, Molecular Ecology, the journal, next generation sequencing, phylogenetics, population genetics, theory | Tagged , , , | 3 Comments

Analysis of the human microbiome reveals you are (at least related to) what you eat, in a manner of speaking

Understanding microbial symbioses, and more specifically how the human microbiome affects our health, is currently a hot topic in the land of microbiology and metagenomics. The most recent special edition of Science focuses on reviews and articles centered on understanding … Continue reading

Posted in genomics, medicine, microbiology, next generation sequencing, population genetics | Leave a comment

Results of the Molecular Ecologist’s Survey on High-Throughput Sequencing

Some days ago, we asked our readers to fill in a survey (now closed) on your use of high-throughput sequencing techniques. We got a lot of responses, a total of 260 people filled in the form. Thank you! Here are the results of your answers. The … Continue reading

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hyRAD and museum genomics

While the RAPTURE may have arrived, the development of novel restriction digest-based library prepartation techniques — and portmanteaus — continues unabated. In a paper published in PLoS ONE last month (and previously available as a preprint on bioRxiv), Tomasz Suchan … Continue reading

Posted in genomics, methods, natural history, next generation sequencing, phylogenetics, phylogeography, population genetics | Tagged , , , , , | Leave a comment