Category Archives: next generation sequencing

Gene expression shows how a plant and its mutualists are better together

No living thing is an island, and many of the encounters between living things that happen every day are not antagonistic or even indifferent, but mutually beneficial. Two such mutualisms that could be among the most important on the planet … Continue reading

Posted in microbiology, next generation sequencing, plants, RNAseq | Tagged , , | Leave a comment

Building bridges across the chaos

In a new review, Eldon and co-authors (in press) attempt to build a bridge across the chaos of genetic patchiness in the sea. They i) describe the patterns characterized as chaotic genetic patchiness, ii) discuss the potential causes of these patterns and … Continue reading

Posted in evolution, genomics, mutation, natural history, next generation sequencing, phylogenetics, phylogeography, population genetics, selection | Tagged , , , | Leave a comment

Is equilibrium out of reach or are there some sneaky bouts of sex?

Reproductive systems impact the evolution of genetic diversity at the population level. Yet, we don’t know a lot about organisms that are partially clonal, despite the large component of biodiversity that dabbles in asexual reproduction to varying degrees. Clonal dynamics are … Continue reading

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The trouble with PCR duplicates

The sequencing center just sent your lane of Illumina data. You’re excited. Life is great. You begin to process the data. You align the data. You check for PCR duplicates. 50 percent. Half of your data is garbage. Everything is … Continue reading

Posted in bioinformatics, genomics, methods, next generation sequencing | Tagged , , , | 3 Comments

How do Missing Data Impact Phylogenetic Inference with UCEs?

Next-generation sequencing (NGS) has put gobs of sequence data in the hands of molecular biologists, and that data is measurably advancing our prospects for a fully resolved Tree of Life. Nearly simultaneously, however, we have realized that every NGS dataset … Continue reading

Posted in next generation sequencing, phylogenetics | Tagged , | 2 Comments

Molecular Inversion Probes: phylogenomics without the excess?

The onset of the phylogenomic era has revolutionized molecular ecology and systematics, helping resolve relationships throughout the tree of life that have long eluded researchers working with only a handful of loci and morphological data. Phylogenetic studies of nonmodel organisms … Continue reading

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STITCH, in time, could save a lot of array design

A new algorithm for processing DNA sequence data, STITCH, could lower costs for studies of genetic variation within species by reconstructing, or “imputing”, the sequences of individual samples within a larger dataset. The ongoing proliferation of high-throughput (or, ugh, “next … Continue reading

Posted in association genetics, bioinformatics, genomics, methods, next generation sequencing, software | Tagged | Leave a comment