Category Archives: next generation sequencing

New to the genome sequencing $8 menu: Nextera library preps!

Researchers are thrifty. We’re always looking for ways to make our expensive supplies and reagents go the extra mile. This shit has been going on for decades – hell, probably even centuries: I remember when I was a kid and … Continue reading

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Posted in genomics, methods, next generation sequencing | Tagged , , , , | 7 Comments

Species and sensibility

Pante et al. (2014) performed a literature review of marine population connectivity in order to illustrate the biased estimates of connectivity which can result from the failure to recognize an evolutionary-relevant unit, such as a species. When exploring the connectivity … Continue reading

Posted in adaptation, community ecology, conservation, DNA barcoding, natural history, next generation sequencing, phylogenetics, population genetics, speciation, theory | 2 Comments

Totally RAD, Part 2

Restriction site-associated DNA sequencing (RADseq) is quickly becoming the go-to methodology for collecting population genetic data, and the methodological difficulties of a technique that is exploding in popularity are coming along with it. Last month, Stacy pointed you towards a … Continue reading

Posted in genomics, Molecular Ecology views, next generation sequencing, population genetics | Tagged , , | Leave a comment

Transcriptomics in the wild (populations)

The genomics revolution is coming has already come. The past decade has seen countless advances in genomic techniques – many of which are now commonly found in any molecular ecologist’s toolbox. For example, instead of measuring gene expression in one … Continue reading

Posted in genomics, next generation sequencing | Tagged , , | 1 Comment

Totally RAD

Puritz et al. (2014) weigh the pros and cons of, the aptly titled, “RAD fad” in a comment recently published online in Molecular Ecology. They challenge: (1) the assertion that the original RAD protocol minimizes the impact of PCR artifacts … Continue reading

Posted in bioinformatics, genomics, methods, next generation sequencing, Uncategorized | 2 Comments

Caught sweeping ‘cross the sea

  The salmon louse Lepeophtheirus salmonis is an ectoparasite linked to declines in wild salmonid populations as well as causing huge economic losses in salmon farms. Previous studies, using a variety of molecular markers, yielded conflicting results ranging from strong … Continue reading

Posted in adaptation, genomics, mutation, next generation sequencing | Leave a comment

From cats to rats: two studies on domestication and tameness

Anyone who has ever read Charles Darwin is acutely aware of his fascination with domestication – particularly how he fancied fancy pigeons. Darwin drew on his domestication obsession while writing his book, The Variation of Animals and Plants under Domestication, … Continue reading

Posted in adaptation, association genetics, domestication, genomics, methods, next generation sequencing, phylogenetics, quantitative genetics | Tagged , , , , , | Leave a comment

The forest resounding at rare intervals with the note of … reproductive isolation

Hybrid zones are often used as a window with which to gaze upon the evolutionary process (Barton and Hewitt 1989). With the advent of genomic tools, it is possible to detect the genomic signatures and the architecture underlying reproductive isolation. In … Continue reading

Posted in adaptation, conservation, genomics, next generation sequencing, population genetics, speciation | 1 Comment

WTF (What’s The Function?)

Jay Shendure’s editorial, “Life after genetics”, points out that we, as geneticists, should shift our focus from variant-finding (e.g., GWAS) to understanding the functional implications of disease-associated variants: “We are in a period of rich discovery in human genetics and genomics. The … Continue reading

Posted in genomics, medicine, mutation, next generation sequencing | Tagged , , , | Leave a comment

How many markers does it take to make a dataset “genomic”?

A new paper in Ecology Letters by Matthew Fitzpatrick and Stephen Keller proposes to use some a class of statistical methods developed for understanding the distribution of species in different environments to understand the distribution of genetic variants in different … Continue reading

Posted in association genetics, genomics, next generation sequencing, population genetics, software | 6 Comments