Category Archives: next generation sequencing

Analysis of the human microbiome reveals you are (at least related to) what you eat, in a manner of speaking

Understanding microbial symbioses, and more specifically how the human microbiome affects our health, is currently a hot topic in the land of microbiology and metagenomics. The most recent special edition of Science focuses on reviews and articles centered on understanding … Continue reading

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Posted in genomics, medicine, microbiology, next generation sequencing, population genetics | Leave a comment

Results of the Molecular Ecologist’s Survey on High-Throughput Sequencing

Some days ago, we asked our readers to fill in a survey (now closed) on your use of high-throughput sequencing techniques. We got a lot of responses, a total of 260 people filled in the form. Thank you! Here are the results of your answers. The … Continue reading

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hyRAD and museum genomics

While the RAPTURE may have arrived, the development of novel restriction digest-based library prepartation techniques — and portmanteaus — continues unabated. In a paper published in PLoS ONE last month (and previously available as a preprint on bioRxiv), Tomasz Suchan … Continue reading

Posted in genomics, methods, natural history, next generation sequencing, phylogenetics, phylogeography, population genetics | Tagged , , , , , | Leave a comment

The Molecular Ecologist’s Survey on High-Throughput Sequencing

Last week we updated the Field Guide to Next Generation DNA Sequencers to better reflect the sequencing techniques of 2016. The NGS Field Guide is one of the most popular resources on the Molecular Ecologist web site, but we don’t know much about how our readers use … Continue reading

Posted in bioinformatics, genomics, methods, next generation sequencing | Tagged , , , , | 2 Comments

The why’s of sex

Sex isn’t quite what it seems – while superficially wasteful in an evolutionary sense (why inherit on only one half of your genes, when you can inherit all of them asexually, or why waste resources in mating when you don’t … Continue reading

Posted in adaptation, evolution, genomics, mutation, natural history, next generation sequencing, population genetics, selection, theory, yeast | Tagged , , , , | Leave a comment

To find duplicated loci in vertebrate polyploids, try thinking small

Big sequencing efforts have gone a long way to help understand the complexities of polyploidy. However, the bioinformatic approaches to sorting and scoring alleles in next-gen data are generally designed for easy of use in diploid species. Unlike a diploid species, where … Continue reading

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RADseq vs. UCEs, round 3

Though reduced-representation genome sequencing (or high-throughput, or nextgen, or massively parallel sequencing, or…) has become standard practice for molecular ecology labs over the past few years, the relative merits of different library preparation methods remains an active area of research. … Continue reading

Posted in bioinformatics, evolution, genomics, methods, next generation sequencing, phylogenetics | Tagged , , , | 1 Comment