Category Archives: methods

Coral conservation through assisted evolution

Coral reefs occupy a tiny portion of the world’s oceans (see map below) but their biodiversity is hugely disproportionate to their size. More than 450 million people from 109 countries live in close proximity to coral reefs and depend upon the … Continue reading

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Posted in adaptation, conservation, evolution, methods | Leave a comment

Show me the power

Describing the patterns of genetic structure and mating system variation in presents challenges from the outset of sample collection to data analysis (see this post and this post). At the beginning of February, I had the pleasure to collaborate with Sean … Continue reading

Posted in conservation, evolution, genomics, interview, methods, population genetics, software, Uncategorized | Leave a comment

The results are in for the journal selection survey

Two weeks ago I wrote a post about a recent paper by Salinas and Munch that presented a model-based method for determining to which journal an author should submit a manuscript for publication. I was curious to know how the readers … Continue reading

Posted in career, Impact Factors, methods, peer review, science publishing | 1 Comment

Genome-wide effects of artificial selection

Humans have been artificially selecting for favorable traits in crops, pets, and livestock over millennia. Years of theoretical predictions and experimental evolution studies have shown the detrimental effects of increased homozygosity, and the population-wide advantages of artificially maintaining heterozygosity. Two … Continue reading

Posted in bioinformatics, evolution, genomics, methods, mutation, population genetics, theory | Leave a comment

Breaking free of the guide tree: two new species delimitation methods

A couple of weeks ago I wrote about a new method to incorporate morphology and DNA sequences into species delimitation. Including both data types improved the results but a couple of tricky spots remained: 1) correctly assigning individuals to putative species and 2) estimating … Continue reading

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Incorporating phenotype and genotype in model-based species delimitation

  Species are the fundamental unit of biology but identifying them is a challenging task that receives a lot of theoretical and empirical attention. In a recent Evolution paper, Solís‐Lemus et al. (2015) introduce a new model-based method that integrates phenotypic and genetic data … Continue reading

Posted in methods, speciation, species delimitation, theory | 1 Comment

New to the genome sequencing $8 menu: Nextera library preps!

Researchers are thrifty. We’re always looking for ways to make our expensive supplies and reagents go the extra mile. This shit has been going on for decades – hell, probably even centuries: I remember when I was a kid and … Continue reading

Posted in genomics, methods, next generation sequencing | Tagged , , , , | 7 Comments

A population genetic R-evolution

Uphill, both ways, in the snow, without shoes … quite apt when thinking of the dark days, in the not too distant past, in which a separate input file was needed for each popgen analysis in order to use a … Continue reading

Posted in howto, methods, population genetics, R, software, Uncategorized | Leave a comment

Linking gene expression and phenotype in an emerging model organism

Last week in his post “Transcriptomics in the wild (populations),” TME contributor Noah Snyder-Mackler focused on a recent paper by Alvarez et al. that reviews the last decade of transcriptomic research including the goal of linking gene expression and phenotype. Researchers today routinely collect transcriptomic data for non-model … Continue reading

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Totally RAD

Puritz et al. (2014) weigh the pros and cons of, the aptly titled, “RAD fad” in a comment recently published online in Molecular Ecology. They challenge: (1) the assertion that the original RAD protocol minimizes the impact of PCR artifacts … Continue reading

Posted in bioinformatics, genomics, methods, next generation sequencing, Uncategorized | 3 Comments