Category Archives: methods

Catching evolution in the act with the Singleton Density Score

A recent study led by Jonathan K. Pritchard at Stanford University brought a media storm with catchy headlines in both of the flagship scientific outlets Nature and Science News. Aside from highlighting the question of preprints without peer review being … Continue reading

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Posted in methods, mutation, population genetics, selection | Tagged , , , | 1 Comment

Opening Pandora’s box: PSMC and population structure

Essentially, all models are wrong, but some are useful. — George Box Publication of the Li and Durbin’s 2011 paper titled “Inference of human population history from individual whole-genome sequences” was a milestone in the inference of demography. By allowing … Continue reading

Posted in bioinformatics, methods, Paleogenomics, population genetics, theory | Tagged , , , , | 3 Comments

RADseq and missing data: some considerations

Unlike Sanger sequencing, where loci are directly targeted for each individual and sequencing errors are relatively rare, massively multilocus datasets from next generation sequencing platforms are characterized by large amounts of missing data. This is particularly true for restriction digest … Continue reading

Posted in bioinformatics, genomics, methods, Molecular Ecology, the journal, next generation sequencing, phylogenetics, population genetics, theory | Tagged , , , | 3 Comments

On Integrative Species Delimitation…

Accurate delimitation of species is a fundamental first step that underlies much of what we do in biology. But this can prove challenging in many situations. Why? Let me count the ways. Incomplete lineage sorting, hybridization, morphological conservatism, and niche … Continue reading

Posted in evolution, methods, phylogeography, population genetics, software, species delimitation | Leave a comment

hyRAD and museum genomics

While the RAPTURE may have arrived, the development of novel restriction digest-based library prepartation techniques — and portmanteaus — continues unabated. In a paper published in PLoS ONE last month (and previously available as a preprint on bioRxiv), Tomasz Suchan … Continue reading

Posted in genomics, methods, natural history, next generation sequencing, phylogenetics, phylogeography, population genetics | Tagged , , , , , | Leave a comment

The Molecular Ecologist’s Survey on High-Throughput Sequencing

Last week we updated the Field Guide to Next Generation DNA Sequencers to better reflect the sequencing techniques of 2016. The NGS Field Guide is one of the most popular resources on the Molecular Ecologist web site, but we don’t know much about how our readers use … Continue reading

Posted in bioinformatics, genomics, methods, next generation sequencing | Tagged , , , , | 2 Comments

The 2016 Next-Generation Sequencing Field Guide Preview: Zombie Systems and New Hope

After a year of minimal activity, we finally have some significant changes in Next Gen Land. In the 2016 update of the NGS Field Guide, I will continue to give my overall interpretation about the various instruments, but with less … Continue reading

Posted in genomics, howto, methods, RNAseq, transcriptomics | Tagged , , , | 6 Comments