Category Archives: methods

STITCH, in time, could save a lot of array design

A new algorithm for processing DNA sequence data, STITCH, could lower costs for studies of genetic variation within species by reconstructing, or “imputing”, the sequences of individual samples within a larger dataset. The ongoing proliferation of high-throughput (or, ugh, “next … Continue reading

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Posted in association genetics, bioinformatics, genomics, methods, next generation sequencing, software | Tagged | Leave a comment

Data, data everywhere and another tool to use: Taxonomer, a web-tool for metagenomics data analysis

Because sequencing. With all the affordable genome and metagenome sequencing available, we’ve reached an unprecedented point at which we can profile microbial communities more accurately than ever before. For this reason, it’s essential to develop efficient methods for data analysis. … Continue reading

Posted in bioinformatics, community ecology, genomics, metagenomics, methods, microbiology, software | Tagged , , , | Leave a comment

Catching evolution in the act with the Singleton Density Score

A recent study led by Jonathan K. Pritchard at Stanford University brought a media storm with catchy headlines in both of the flagship scientific outlets Nature and Science News. Aside from highlighting the question of preprints without peer review being … Continue reading

Posted in methods, mutation, population genetics, selection | Tagged , , , | 1 Comment

Opening Pandora’s box: PSMC and population structure

Essentially, all models are wrong, but some are useful. — George Box Publication of the Li and Durbin’s 2011 paper titled “Inference of human population history from individual whole-genome sequences” was a milestone in the inference of demography. By allowing … Continue reading

Posted in bioinformatics, methods, Paleogenomics, population genetics, theory | Tagged , , , , | 3 Comments

RADseq and missing data: some considerations

Unlike Sanger sequencing, where loci are directly targeted for each individual and sequencing errors are relatively rare, massively multilocus datasets from next generation sequencing platforms are characterized by large amounts of missing data. This is particularly true for restriction digest … Continue reading

Posted in bioinformatics, genomics, methods, Molecular Ecology, the journal, next generation sequencing, phylogenetics, population genetics, theory | Tagged , , , | 5 Comments

On Integrative Species Delimitation…

Accurate delimitation of species is a fundamental first step that underlies much of what we do in biology. But this can prove challenging in many situations. Why? Let me count the ways. Incomplete lineage sorting, hybridization, morphological conservatism, and niche … Continue reading

Posted in evolution, methods, phylogeography, population genetics, software, species delimitation | Leave a comment

hyRAD and museum genomics

While the RAPTURE may have arrived, the development of novel restriction digest-based library prepartation techniques — and portmanteaus — continues unabated. In a paper published in PLoS ONE last month (and previously available as a preprint on bioRxiv), Tomasz Suchan … Continue reading

Posted in genomics, methods, natural history, next generation sequencing, phylogenetics, phylogeography, population genetics | Tagged , , , , , | Leave a comment