Category Archives: methods

The trouble with PCR duplicates

The sequencing center just sent your lane of Illumina data. You’re excited. Life is great. You begin to process the data. You align the data. You check for PCR duplicates. 50 percent. Half of your data is garbage. Everything is … Continue reading

Posted in bioinformatics, genomics, methods, next generation sequencing | Tagged , , , | 2 Comments

On (mis)interpreting STRUCTURE/ADMIXTURE results

STRUCTURE, ADMIXTURE and other similar software are among the most cited programs in modern population genomics. They are algorithms that estimate allele frequencies and admixture proportions under the premise that sampled genotypes are derived from one of “K” ancestral populations, … Continue reading

Posted in bioinformatics, genomics, howto, methods, population genetics, software, STRUCTURE | Tagged , , , , | Leave a comment

Understanding diverse microbial communities: An interview with A. Murat Eren (Meren)

It’s clear that microbes play a crucial role in practically every aspect of ecosystems globally. From the deepest, most remote and unexplored regions of the ocean, to the human oral cavity, there are diverse microbial assemblages driving Earth’s biogeochemical cycles. … Continue reading

Posted in bioinformatics, community ecology, metagenomics, methods | Tagged , , , | 2 Comments

STITCH, in time, could save a lot of array design

A new algorithm for processing DNA sequence data, STITCH, could lower costs for studies of genetic variation within species by reconstructing, or “imputing”, the sequences of individual samples within a larger dataset. The ongoing proliferation of high-throughput (or, ugh, “next … Continue reading

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Data, data everywhere and another tool to use: Taxonomer, a web-tool for metagenomics data analysis

Because sequencing. With all the affordable genome and metagenome sequencing available, we’ve reached an unprecedented point at which we can profile microbial communities more accurately than ever before. For this reason, it’s essential to develop efficient methods for data analysis. … Continue reading

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Catching evolution in the act with the Singleton Density Score

A recent study led by Jonathan K. Pritchard at Stanford University brought a media storm with catchy headlines in both of the flagship scientific outlets Nature and Science News. Aside from highlighting the question of preprints without peer review being … Continue reading

Posted in methods, mutation, population genetics, selection | Tagged , , , | 1 Comment

Opening Pandora’s box: PSMC and population structure

Essentially, all models are wrong, but some are useful. — George Box Publication of the Li and Durbin’s 2011 paper titled “Inference of human population history from individual whole-genome sequences” was a milestone in the inference of demography. By allowing … Continue reading

Posted in bioinformatics, methods, Paleogenomics, population genetics, theory | Tagged , , , , | 3 Comments