Category Archives: bioinformatics

PCA of multilocus genotypes in R

An earlier post from Mark Christie showed up on my feed on calculating allele frequencies from genotypic data in R, and I wanted to put together a quick tutorial on making PCA (Principal Components Analysis) plots using genotypes. I used … Continue reading

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Posted in bioinformatics, genomics, howto, population genetics, R, software | Tagged , , | 1 Comment

What do with all those pesky mtDNA reads in your NGS experiment

Have you ever noticed how many reads from your high throughput sequencing project map to the tiny fraction of your genome that is the mitochondrial genome (mtDNA)? Pretty much any NGS experiment (e.g., RNA-seq, DNA-seq, capture-based sequencing) leave you with … Continue reading

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IMa2p – Parallel Isolation with Migration Analyses

I figured that it was time to write an update on my post from a year ago on Bayesian MCMC in inferring ancestral demography. Recently, my postdoctoral advisor, Jody Hey and I released a version of the popular IMa2 program, … Continue reading

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Mixed modeling of methylation measures (increase your power by 60%)

Do you want to increase your power to detect differentially methylated CpG sites by 60%*? Yes?! Then do I have the pre-print for you.

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IBE/IBD Contour plots in R

Rob’s post from yesterday motivated me to find an alternate way of visualizing correlations between matrices of geographical or ecological data, and genetic data. I have seen plenty of Mantel, or partial Mantel tests of correlation, as well as plots … Continue reading

Posted in bioinformatics, howto, population genetics, R, software | Tagged , , , | 5 Comments

Another lesson in genomics experimental design and avoiding batch effects

Twitter has been abuzz with Orna Man and Yoav Gilad’s (re)analysis of the data from a recent PNAS paper: “Comparison of the transcriptional landscapes between human and mouse tissues”. The PNAS paper concluded that the gene expression profiles of different … Continue reading

Posted in bioinformatics, genomics, next generation sequencing, RNAseq | Tagged , , | 2 Comments

When genomes duplicate

Whole genome duplication events have played an important role in the evolutionary history of plants. Vallejo-Marín et al. (2015) describe origins of a new polyploid species, Mimulus peregrines, found on the Scottish mainland as well as the Orkney Islands. It was formed within … Continue reading

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Sous les mers: cradles or museums of biodiversity?

While thinking about environmental genomics and writing this post on a recent article in Heredity, I interviewed Eric Pante.

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Grasping gorgonians

A recent issue of Heredity focused on the brave new world of environmental genomics. After highlighting the special issue, I started chatting to one of the contributors, Eric Pante and became interested in his work on gorgonians. Eric and his co-authors explored the … Continue reading

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The brave new world of environmental genomics

A new special issue of Heredity reflects on the recent advances in environmental genomics (see other posts about eDNA here and here) and highlights the ways NGS can aid in characterizing complex biological systems. The cryptic, as well as the rare but active … Continue reading

Posted in bioinformatics, Coevolution, DNA barcoding, evolution, genomics | Tagged , , , | 1 Comment